![]() ![]() It can be used on PVDF and nylon membrane. It contains 2 reference bands ~25 and ~75 kDa coupled with blue chromophore as well as red dye and green dye for easy identification. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. For silver staining the amount of Protein Ladder applied might be decreased up to 10 times.Tricolor Broad Range Prestained Protein Ladder contains 13 proteins that resolve into sharp, tight bands in the range of 5-245kDa.It can be used to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. The Protein Ladder is optimized for use with Coomassie Brilliant Blue R-250 staining. The 200kDa band has lower intensity than the other bands when detected on Western blots using Strep-Tactin® -AP conjugate. When Western blots are performed, Prestained Protein Ladder (#65-0671) might be used for monitoring of transfer efficiency. If thicker gels are used, the loading volume should be increased. Precision Plus Protein Unstained Standards are a mixture of ten Strep-tagged, recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). This standard allows accurate molecular weight determination when. When Western blots are indicated for in gels with a thickness of 0.75mm. The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 (1,2) or other commercially available, ready-to-use protein stains. If thicker gels are used, the loading volume should be increased. Loading volumes are indicated for in gels with a thickness of 0.75mm. The 50k Da protein band is of greater intensity and serves as a reference. After the run is complete, stain the gel or perform Load the Ladder on an SDS-PAA gel and run.Ĥ. After transferring the protein bands onto the blot paper, stain the paper. The ladder is visualized by SDS-PAGE using coomassie or silver stains or detected in Western blots with protein stains. If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do. Mix gently, but thoroughly to ensure the solution is homogeneous.ģ. The Thermo Scientific PageRuler Unstained Protein Ladder consists of a unstained mixture of 14 recombinant, highly purified proteins ranging from 10 kDa to 200 kDa. Thaw the marker at room temperature or heat at 37-40℃ for a few minutes to dissolve precipitated solids. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on. ![]() Precise molecular weight determination of polypeptides in denaturing PAA gels and Western blots (1, 4).ġ. Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. ![]() Each protein contains an integral Strep-tag® II sequence and may be detected on Western blots using Strep-Tactin® -AP conjugate or antibody against Strep-tag® II (2,3).Īpproximately 0.02-0.05mg/ml of each protein in the following storage buffer (62.5mM Tris-H3PO4 (pH 7.5 at 25), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol). The Protein Ladder is supplied in gel loading buffer and is ready-to-use. The proteins resolve into clearly identifiable sharp bands from 10kDa to 200kDa when analyzed by SDS-PAGE and stained with Coomassie Blue (1). The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Protein Ladder, 10-200kDa, is a mixture of 14 recombinant, highly purified proteins designed for precise sizing of proteins by SDS-PAGE. HiMark Unstained High Molecular Weight (HMW) Protein Standard contains nine proteins in the range of 40500 kDa designed for analysis of high molecular weight proteins. ![]()
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